Comparison of gizzard and intestinal microbiota of wild neotropical birds

• DOI: 10.1371/journal.pone.0194857
• Publication Date: 2018-03-26T17:38:51Z
• Authors: M. Alexandra Garc...; Hakdong Shin; Virginia Sanz; Miguel Lentino; L. Margarita Mart...; Monica Contreras; Fabian Michelangeli; María Gloria Domí...
• Keywords: DNA, Bacterial; 0301 basic medicine; Principal Component Analysis; Bacteria; Science; Q; R; Stomach Diseases; Columbiformes; Discriminant Analysis; Animals, Wild; Biodiversity; Sequence Analysis, DNA; 15. Life on land; Gastrointestinal Microbiome; Intestines; 03 medical and health sciences; RNA, Ribosomal, 16S; Gizzard, Avian; Medicine; Animals; Passeriformes; Algorithms; Research Article

Gut bacterial communities have been shown to be influenced by diet, host phylogeny and anatomy, but most of these studies have been done in captive animals. Here we compare the bacterial communities in the digestive tract of wild birds. We characterized the gizzard and intestinal microbiota among 8 wild Neotropical bird species, granivorous or frugivorous species of the orders Columbiformes and Passeriformes. We sequenced the V4 region of the 16S rRNA gene in 94 collected samples from 32 wild birds from 5 localities, and compared bacterial communities by foraging guild, organ, locality and bird taxonomy. 16S rRNA gene-based sequencing data were examined using QIIME with linear discriminant analysis effect size (LEfSe) and metabolic pathways were predicted using PICRUSt algorism. We identified 8 bacterial phyla, dominated by Firmicutes, Actinobacteria and Proteobacteria. Beta diversity analyses indicated significant separation of gut communities by bird orders (Columbiformes vs. Passerifomes) and between bird species (p<0.01). In lower intestine, PICRUSt shows a predominance of carbohydrate metabolism in granivorous birds and xenobiotics biodegradation pathways in frugivorous birds. Gizzard microbiota was significantly richer in granivorous, in relation to frugivorous birds (Chao 1; non-parametric t-test, p<0.05), suggesting a microbial gizzard function, beyond grinding food. The results suggest that the most important factor separating the bacterial community structure was bird taxonomy, followed by foraging guild. However, variation between localities is also likely to be important, but this could not been assessed with our study design.