Salmonella-vectored vaccine delivering three Clostridium perfringens antigens protects poultry against necrotic enteritis

• DOI: 10.1371/journal.pone.0197721
• Publication Date: 2019-02-12T18:42:45Z
• Authors: Shyra Wilde; Yanlong Jiang; Amanda M. Tafoya; Jamie Horsman; Miranda Yousif; Luis Armando Vazquez; Kenneth L. Roland
• Keywords: Clostridium perfringens; Salmonella enterica; Toxoid

Necrotic enteritis is an economically important poultry disease caused by the bacterium Clostridium perfringens. There are currently no necrotic vaccines commercially available for use in broiler birds, most target population. Salmonella-vectored represent a convenient and effective option controlling this disease. We used single attenuated Salmonella vaccine strain, engineered to lyse within host, deliver up three C. perfringens antigens. Two of antigens were toxoids, based on α-toxin NetB toxin. The third antigen was fructose-1,6-bisphosphate aldolase (Fba), metabolic enzyme with unknown role virulence. Oral immunization strain producing either Fba, α-toxoid toxoid, or all antigens, immunogenic, inducing serum, cellular mucosal responses against vectored All strains partially protective virulent challenge. delivering Fba only provided best protection. also demonstrate that both toxins present cell surface. presence surface suggests may function as adhesin.