Identification of attractive odorants released by preferred bacterial food found in the natural habitats of C. elegans

• DOI: 10.1371/journal.pone.0201158
• Publication Date: 2018-07-23T17:30:08Z
• Authors: Soleil E. Worthy; Lillian Haynes; Melissa Chambers; Danika Bethune; Emily Kan; Kevin Chung; Ryan Ota; Charles J. Taylor; Elizabeth E. Glater
• Keywords: 0301 basic medicine; Volatile Organic Compounds; Bacteria; Science; Q; R; Choice Behavior; Gas Chromatography-Mass Spectrometry; Food Preferences; 03 medical and health sciences; Pentanols; Physical Stimulation; Predatory Behavior; Odorants; Medicine; Animals; Caenorhabditis elegans; Ecosystem; Research Article

Food choice is critical for survival because organisms must choose food that is edible and nutritious and avoid pathogenic food. Many organisms, including the nematode C. elegans, use olfaction to detect and distinguish among food sources. C. elegans exhibits innate preferences for the odors of different bacterial species. However, little is known about the preferences of C. elegans for bacterial strains isolated from their natural environment as well as the attractive volatile compounds released by preferred natural bacteria isolates. We tested food odor preferences of C. elegans for non-pathogenic bacteria found in their natural habitats. We found that C. elegans showed a preference for the odor of six of the eight tested bacterial isolates over its standard food source, E. coli HB101. Using solid-phase microextraction and gas chromatography coupled with mass spectrometry, we found that four of six attractive bacterial isolates (Alcaligenes sp. JUb4, Providenica sp. JUb5, Providencia sp. JUb39, and Flavobacteria sp. JUb43) released isoamyl alcohol, a well-studied C. elegans attractant, while both non-attractive isolates (Raoultella sp. JUb38 and Acinetobacter sp. JUb68) released very low or non-detectable amounts of isoamyl alcohol. In conclusion, we find that isoamyl alcohol is likely an ethologically relevant odor that is released by some attractive bacterial isolates in the natural environment of C. elegans.