Physiological analysis of the effect of altitudinal gradients on Leymus secalinus on the Qinghai-Tibetan Plateau

• DOI: 10.1371/journal.pone.0202881
• Publication Date: 2018-09-05T13:41:51Z
• Authors: Guowen Cui; Bing Li; Wenhua He; Xiujie Yin; Shengyong Liu; Lu Lian; Yaling Zhang; Wenxue Liang; Pan Zhang
• Keywords: Chlorophyll; 0106 biological sciences; Proline; Superoxide Dismutase; Science; Altitude; Q; R; 15. Life on land; Catalase; Poaceae; Tibet; Carotenoids; 01 natural sciences; Peroxides; Plant Leaves; Medicine; Lipid Peroxidation; Sugars; Research Article; Plant Proteins

On the Qinghai-Tibetan Plateau, the high-altitudinal gradients can negatively affect plant distribution and limit plant growth and reproduction. Leymus secalinus (Georgi) Tzvel. is an important forage crop on the Qinghai-Tibetan Plateau and has an excellent ability to fix sand and improve soil. To evaluate the effect of altitude on the physiological characteristics of L. secalinus on the Qinghai-Tibetan Plateau, we measured the lipid peroxidation; chlorophyll a (Chl a), chlorophyll b (Chl b), total carotenoid (Car), soluble protein, proline and soluble sugar contents; and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in leaves from eight different altitudes in Minhe County and Huangzhong County. The leaves were collected at the initial bloom stage, and the average vertical distance between two adjacent collection sites was approximately 100 meters. The reduction in Chl a and Chl b contents and the increase in Car contents can allow plants to weaken their light absorption and avoid photodamage to the chloroplast. The decrease in malondialdehyde (MDA) content associated with lower lipid peroxidation, and the changes of CAT, SOD and POD activities reflect a higher reactive oxygen species (ROS) scavenging capacity in high-altitude plants. The increase in proline and soluble sugar contents with elevation suggests that proline and soluble sugar may play a key role in the osmotic adjustment of plants in alpine regions. The results suggested that altitudinal gradients negatively affect L. secalinus on the Qinghai-Tibetan Plateau and that the adaptation mechanism and survival strategies of L. secalinus were attributed to the combined effects of multiple protective strategies.