Novel urinary exosomal biomarkers of acute T cell-mediated rejection in kidney transplant recipients: A cross-sectional study

• DOI: 10.1371/journal.pone.0204204
• Publication Date: 2018-09-18T17:54:02Z
• Authors: Jeong-Hoon Lim; Chan-Hyeong Lee; Kyu Yeun Kim; Hee-Yeon Jung; Ji-Young Choi; Jang-Hee Cho; Sun-Hee Park; Yong-Lim Kim; Moon-Chang Baek; Jae Berm Park; Young-Hoon Kim; Byung Ha Chung; Sang-Ho Lee; Chan-Duck Kim
• Keywords: Adult; Graft Rejection; Male; Proteomics; Tetraspanins; Science; T-Lymphocytes; Exosomes; Sensitivity and Specificity; 03 medical and health sciences; Hemopexin; Tandem Mass Spectrometry; Humans; 0303 health sciences; Q; R; Middle Aged; Kidney Transplantation; 3. Good health; Cross-Sectional Studies; Early Diagnosis; Gene Expression Regulation; Medicine; Female; Biomarkers; Research Article; Chromatography, Liquid

Acute rejection is hazardous to graft survival in kidney transplant recipients (KTRs). We aimed to identify novel biomarkers for early diagnosis of acute T cell-mediated rejection (TCMR) in urinary exosomes of KTRs.Among 458 graft biopsies enrolled in a cross-sectional multicenter study, 22 patients with stable graft function (STA) who had not shown pathologic abnormality and 25 patients who diagnosed biopsy-proven TCMR were analyzed. We performed proteomic analysis using nano-ultra performance liquid chromatography-tandem mass spectrometry (nano-UPLC-MS/MS) to identify candidate biomarkers for early TCMR diagnosis on urinary exosomes. We confirmed the protein levels of each candidate biomarker by western blot analysis.A total of 169 urinary exosome proteins were identified by nano-UPLC-MS/MS. Forty-six proteins showed increased expression in STA patients, while 17 proteins were increased in TCMR patients. Among them, we selected five proteins as candidate biomarkers for early diagnosis of TCMR according to significance, degree of quantity variance, and information from the ExoCarta database. We confirmed the proteomic expression levels of five candidate biomarkers by western blot analysis in each patient. Of all candidate biomarkers, tetraspanin-1 and hemopexin were significantly higher in TCMR patients (STA:TCMR ratio = 1:1.8, P = 0.009, and 1:3.5, P = 0.046, respectively).Tetraspanin-1 and hemopexin were detected in KTR urine and could act as potential diagnostic proteins for TCMR.