MYB1 transcription factor is a candidate responsible for red root skin in radish (Raphanus sativus L.)

• DOI: 10.1371/journal.pone.0204241
• Publication Date: 2018-09-21T18:17:55Z
• Authors: Gibum Yi; June-Sik Kim; Jeong Eun Park; Hosub Shin; Seung Hwa Yu; Suhyung Park; Jin Hoe Huh
• Keywords: Raphanus; White (mutation)

Root skin color is one of the economically important traits in radish (Raphanus sativus), and pigmentation red varieties largely attributable to anthocyanin accumulation. Pelargonidin was found as a major pigment accumulated sub-epidermal layer roots. In 20 F2 population generated from F1 with root skins, skins white colors segregated 3:1 ratio. Additionally, test cross between F3 individual gave rise 1:1 segregation white, indicating that determined by single locus dominant over white. We performed association mapping for using SNPs obtained RNA-seq analysis. Segregation analysis on 152 test-cross revealed an RsMyb1 transcription factor candidate gene determine color. A PCR marker based polymorphism within 2 kb developed tested 12 individuals populations, respectively, were completely distinguished corresponding genotypes. Expression levels or purple cultivars significantly higher than cultivars. These findings suggest crucial determinant biosynthesis roots, molecular this study will be useful marker-assisted selection at early seedling stages.