The role of BMP6 in the proliferation and differentiation of chicken cartilage cells

• DOI: 10.1371/journal.pone.0204384
• Publication Date: 2019-07-01T17:27:17Z
• Authors: Fei Ye; Hengyong Xu; Huadong Yin; Xiaoling Zhao; Diyan Li; Qing Zhu; Yan Wang
• Keywords: 0301 basic medicine; 0303 health sciences; Dose-Response Relationship, Drug; Bone Morphogenetic Protein 6; Science; Q; R; Cell Differentiation; Chick Embryo; Avian Proteins; 03 medical and health sciences; Cartilage; Growth Hormone; Medicine; Animals; RNA, Messenger; Cells, Cultured; Research Article; Cell Proliferation; Signal Transduction

AbstractPrevious studies have indicated that bone morphogenetic protein (BMP) 6 plays an important role in skeletal system development and progression. However, the mechanism underlying the effects of BMP6 in cartilage cell proliferation and differentiation remains unknown. In this study, cartilage cells were isolated from shanks of chicken embryos and treated with different concentrations of GH. Cell proliferation and differentiation potential was assessed using real-time polymerase chain reaction (RT-PCR) and CCK-8 assays in vitro. The results showed that at 48 h, the Collagen II and BMP6 expression levels in 50 ng/μl GH-treated cartilage cells were significantly higher than in groups treated with 100 ng/μl or 200 ng/μl GH. We further observed that knockdown of BMP6 in cartilage cells led to significantly decreased expression levels of Collagen II and Collagen X. Moreover, the suppression of BMP6 expression by a specific siRNA vector led to significantly decreased expression levels of IGF1R, JAK, PKC, PTH, IHH and PTHrP. Taken together, our data suggest that BMP6 may play a critical role in chicken cartilage cell proliferation and differentiation through the regulation of IGF1, JAK2, PKC, PTH, and Ihh-PTHrP signaling pathways.