Apolipoprotein E is a pancreatic extracellular factor that maintains mature β-cell gene expression

• DOI: 10.1371/journal.pone.0204595
• Publication Date: 2018-10-10T18:23:02Z
• Authors: Ahmed I. Mahmoud; Francisco X. Galdos; Katherine A. Dinan; Mark P. Jedrychowski; Jeffrey C. Davis; Ana Vujic; Inbal Rachmin; Christian Shigley; James R. Pancoast; Samuel Lee; Jennifer Holliste...; Catherine M. MacG...; Steven P. Gygi; Douglas A. Melton; Gordon C. Weir; Richard T. Lee
• Keywords: Apolipoprotein E

The in vivo microenvironment of tissues provides myriad unique signals to cells. Thus, following isolation, many cell types change culture, often preserving some but not all their characteristics culture. At least the may be mimicked by providing specific cues cultured Here, we show that after isolation and during maintenance adherent rat islets reduce expression key β-cell transcription factors necessary for function soluble pancreatic decellularized matrix (DCM) can enhance gene expression. Following chromatographic fractionation DCM, performed proteomics identify maintain stability function. We identified Apolipoprotein E (ApoE) as an extracellular protein significantly increased genes. ApoE effect on beta cells was mediated at part through JAK/STAT signaling pathway. Together, these results reveal a role factor mature profile.