Extracellular polymeric substance (EPS)-degrading enzymes reduce staphylococcal surface attachment and biocide resistance on pig skin in vivo

• DOI: 10.1371/journal.pone.0205526
• Publication Date: 2018-10-10T18:44:16Z
• Authors: Jeffrey B. Kaplan; Kevin D. Mlynek; Hashani Hettiarac...; Yonas A. Alamneh; Lionel Biggemann; Daniel V. Zurawski; Chad C. Black; Charles E. Bane; Robert K. Kim; Mark S. Granick
• Keywords: 0301 basic medicine; Staphylococcus aureus; Extracellular Polymeric Substance Matrix; Science; Q; Sus scrofa; R; Bacterial Adhesion; Recombinant Proteins; Anti-Bacterial Agents; 3. Good health; Disease Models, Animal; 03 medical and health sciences; Drug Resistance, Bacterial; Staphylococcus epidermidis; Medicine; Animals; Deoxyribonuclease I; Humans; Female; Staphylococcal Skin Infections; Povidone-Iodine; Research Article

Staphylococcal extracellular polymeric substances (EPS) such as DNA (eDNA) and poly-N-acetylglucosamine surface polysaccharide (PNAG) mediate numerous virulence traits including host colonization antimicrobial resistance. Previous studies showed that EPS-degrading enzymes increase staphylococcal biocide susceptibility in vitro vivo, decrease animal models. In the present study we tested effect of on skin povidone iodine using a novel vivo pig model enabled us to colonize treat 96 isolated areas single vivo. To quantitate colonization, punch biopsies colonized were homogenized, diluted, plated agar for colony forming unit enumeration. Skin was with either Staphylococcus epidermidis or aureus. Two enzymes, DNase I PNAG-degrading enzyme dispersin B, employed. Enzymes their ability inhibit detach preattached bacteria. The S. aureus killing by also measured. We found B significantly inhibited detached cells from skin. A cocktail increased iodine. These findings suggest EPS components eDNA PNAG contribute resistance may be useful adjunct conventional antisepsis procedures order further reduce bioburden.