Characterization of longitudinal canal tissue in the acorn barnacle Amphibalanus amphitrite

• DOI: 10.1371/journal.pone.0208352
• Publication Date: 2018-12-10T19:07:38Z
• Authors: Chenyue Wang; Janna N. Schultzhaus; Chris R. Taitt; Dagmar H. Leary; Lisa C. Shriver-Lake; Daniel Snellings; Samantha Sturiale; Stella H. North; Beatriz Orihuela; Daniel Rittschof; Kathryn J. Wahl; Christopher M. Sp...
• Keywords: Parenchyma; Vitellogenin; Yolk

The morphology and composition of tissue located within parietal shell canals the barnacle Amphibalanus amphitrite are described. Longitudinal canal nearly spans length side plates, terminating near leading edge specimen basis in proximity to female reproductive throughout peripheral sub-mantle region, i.e. mantle parenchyma. Microscopic examination stained longitudinal sections reveal presence cell nuclei as well an abundance micron-sized spheroids staining positive for basic residues lipids. Spheroids with same profile present extensively ovarioles, particularly oocytes which readily identifiable at various developmental stages. Mass spectrometry analysis compared collected from parenchyma reveals a 50% overlap protein greatest number sequence matches vitellogenin, glycolipoprotein playing key role vitellogenesis–yolk formation developing oocytes. morphological similarity tissue, combined mass two tissues, provides compelling evidence that one several possible functions is supporting system A. amphitrite, thus expanding understanding growth development this sessile marine organism.