Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing

• DOI: 10.1371/journal.pone.0211339
• Publication Date: 2019-03-08T21:03:45Z
• Authors: Yosuke Takeoka; Keitaro Matsumoto; Daisuke Taniguchi; Tomoshi Tsuchiya; Ryusuke Machino; Masaaki Moriyama; Shosaburo Oyama; Tomoyuki Tetsuo; Yasuaki Taura; Katsunori Takagi; Takuya Yoshida; Abdelmotagaly Elg...; Naoto Matsuo; Masaki Kunizaki; Shuichi Tobinaga; Takashi Nonaka; Shigekazu Hidaka; Naoya Yamasaki; Koichi Nakayama; Takeshi Nagayasu
• Keywords: Regenerative Medicine

Various strategies have been attempted to replace esophageal defects with natural or artificial substitutes using tissue engineering. However, these methods not yet reached clinical application because of the high risks related their immunogenicity insufficient biocompatibility. In this study, we developed a scaffold-free structure mixture cell types bio-three-dimensional (3D) printing technology and assessed its characteristics in vitro vivo after transplantation into rats. Normal human dermal fibroblasts, smooth muscle cells, bone marrow-derived mesenchymal stem umbilical vein endothelial cells were purchased used as source. After preparation multicellular spheroids, esophageal-like tube structures prepared by bio-3D printing. The matured bioreactor transplanted 10-12-week-old F344 male rats grafts under general anesthesia. Mechanical histochemical assessment performed. Among 4 evaluated, those larger proportion tended show greater strength expansion on mechanical testing highly expressed α-smooth actin vascular growth factor immunohistochemistry. Therefore, was selected for transplantation. had sufficient between esophagus stomach silicon stents. maintained 30 days Smooth maintained, flat epithelium extended covered inner surface lumen. Food also passed through structure. These results suggested that esophagus-like tubular created could hold promise substitute repair defects.