Actin-binding domains mediate the distinct distribution of two Dictyostelium Talins through different affinities to specific subsets of actin filaments during directed cell migration

• DOI: 10.1371/journal.pone.0214736
• Publication Date: 2019-04-04T17:42:05Z
• Authors: Masatsune Tsujioka; Taro Q. P. Uyeda; Yoshiaki Iwadate; Hitesh Patel; Keitaro Shibata; Tenji Yumoto; Shigenobu Yonemura
• Keywords: Lamellipodium; Actin-binding protein

Although the distinct distribution of certain molecules along anterior or posterior edge is essential for directed cell migration, mechanisms to maintain asymmetric protein localization have not yet been fully elucidated. Here, we studied a mechanism localizations two Dictyostelium talin homologues, A and B, both which play important roles in migration adhesion. Using GFP fusion, found that as well its C-terminal actin-binding region, consists an I/LWEQ domain villin headpiece domain, was restricted leading migrating cells. This sharp contrast co-localized with myosin II cortex, reported previously. Intriguingly, even II-null cells, displayed specific distribution, co-localizing stretched actin filaments. In contrast, B excluded from regions rich filaments, although amount region alone present those areas. When cells were sucked by micro-pipette, detected retracting aspirated lobe where acto-myosin, A, accumulated. Based on these results, suggest predominantly interacts filaments through while does make such distinctions. Furthermore, appears additional, unidentified excludes it We propose properties enrichment respectively, during migration.