Bone regeneration in Ds-Red pig calvarial defect using allogenic transplantation of EGFP-pMSCs – A comparison of host cells and seeding cells in the scaffold

• DOI: 10.1371/journal.pone.0215499
• Publication Date: 2019-07-18T13:29:34Z
• Authors: Ming-Kai Hsieh; Chia-Jung Wu; Xuan-Chun Su; Yi-Chen Chen; Tsung-Ting Tsai; Chi-Chien Niu; Po-Liang Lai; Shinn-Chih Wu
• Keywords: 0301 basic medicine; Bone Regeneration; Tissue Scaffolds; Swine; Science; Q; Green Fluorescent Proteins; Skull; R; Mesenchymal Stem Cells; Mesenchymal Stem Cell Transplantation; 03 medical and health sciences; Osteogenesis; Medicine; Animals; Transplantation, Homologous; Cells, Cultured; Research Article

Background Cells, scaffolds, and factors are the triad of regenerative engineering; however, it is difficult to distinguish whether cells in construct from seeded or host via blood supply. We performed a novel vivo study transplant enhanced green fluorescent pig mesenchymal stem (EGFP-pMSCs) into calvarial defect DsRed pigs. The cell distribution proportion were distinguished by different colors through whole period. Method/Results Eight adult domestic Ds-Red pigs treated with five modalities: empty defects without scaffold (group 1); filled only 2); osteoinduction medium-loaded 3); 5 x 103 cells/scaffold 4); 104 5). vitro distribution, morphology, osteogenic differentiation, fluorescence images groups 4 analyzed. Two animals sacrificed at 1, 2, 3, weeks after transplantation. imaging quantification data showed that EGFP-pMSCs represented scaffolds throughout A higher density resulted more sustained bone regeneration compared lower density. Host recruited if enough space was available scaffold. 1 3 did not change 1st week 4th week, which indicates cannot recruit even when for ingrowth. histological immunohistochemical involved osteogenesis cells. Conclusion Our results both types participate osteogenesis. These suggest may be effective