Rapid and highly sensitive detection of pyocyanin biomarker in different Pseudomonas aeruginosa infections using gold nanoparticles modified sensor

• DOI: 10.1371/journal.pone.0216438
• Publication Date: 2019-07-30T17:25:19Z
• Authors: Amal A. Elkhawaga; Marwa M. Khalifa; Omnia El-badawy; Mona A. Hassan; Waleed A. El-Said
• Keywords: Pyocyanin

Successful antibiotic treatment of infections relies on accurate and rapid identification the infectious agents. Pseudomonas aeruginosa is implicated in a wide range human that mostly become complicated life threating, especially immunocompromised critically ill patients. Conventional microbiological methods take more than three days to obtain results. Pyocyanin distinctive electroactive biomarker for aeruginosa. Here, we have prepared polyaniline/gold nanoparticles decorated ITO electrode tested it establish rapid, diagnostic highly sensitive pyocyanin sensor culture clinical isolates with high selectivity traces when measured existence different interferences like vitamin C, uric acid, glucose. The scanning electron microscopy cyclic voltammetry techniques were used characterize morphology electrical conductivity constructed electrode. determined linear detection was from 238 μM 1.9 limit 500 nM. Compared screen-printed before, showed 4-fold enhanced performance. Furthermore, PANI/Au NPs/ITO modified electrodes demonstrated ability detect directly without any potential interference other species.