Enhanced proliferation and differentiation of mesenchymal stem cells by astaxanthin-encapsulated polymeric micelles

• DOI: 10.1371/journal.pone.0216755
• Publication Date: 2019-05-20T17:33:57Z
• Authors: Jun Zhang; Ching-An Peng
• Keywords: Science; Polyesters; Xanthophylls; Antioxidants; Polyethylene Glycols; 03 medical and health sciences; Chondrocytes; Nanocapsules; Chlorophyta; Adipocytes; Humans; Cells, Cultured; Micelles; Cell Proliferation; Drug Carriers; 0303 health sciences; Osteoblasts; Q; R; Cell Differentiation; Mesenchymal Stem Cells; Drug Liberation; Solubility; Medicine; Research Article

Astaxanthin is a highly potent antioxidant which can be extracted from Haematococcus pluvialis when cultivated and induced at high stress conditions. Due to astaxanthin's hydrophobicity, methoxypolyethylene glycol-polycaprolactone (mPEG-PCL) copolymer was synthesized form polymeric micelles for the encapsulation of astaxanthin. Astaxanthin-loaded were then used examine effects on proliferation differentiation human mesenchymal stem cells (MSCs). Dynamic light scattering (DLS) Fourier transform infrared spectroscopy (FT-IR) confirmed astaxanthin encapsulated into mPEG-PCL micelles. loading efficiency, determined by UV/Vis spectroscopy, 3.27% 96.67%, respectively. After 48 h, total 87.31% released The drug release profile better fit Michaelis-Menten type model than power law model. MSC culture results showed that medium supplemented with 0.5 μg/mL astaxanthin-encapsulated led 26.3% increase in over an 8-day period. 20 ng/mL enhanced adipogenesis, chondrogenesis, osteogenesis MSCs 52%, 106%, 182%,