Sub-genotype phylogeny of the non-G, non-P genes of genotype 2 Rotavirus A strains

• DOI: 10.1371/journal.pone.0217422
• Publication Date: 2019-05-31T13:38:56Z
• Authors: Chantal Ama Agbem...; Toyoko Nakagomi; Susan Afua Damanka; Francis Ekow Dennis; Belinda Larteley ...; George Enyimah Armah; Osamu Nakagomi
• Keywords: Reassortment; Lineage (genetic)

Recent increase in the detection of unusual G1P[8], G3P[8], G8P[8], and G9P[4] Rotavirus A (RVA) strains bearing DS-1-like constellation non-G, non-P genes (hereafter referred to as genotype 2 backbone) requires better understanding their evolutionary relationship. However, within a genotype, there is lack consensus lineage designation framework set common sequences that can serve references. Phylogenetic analyses were carried out on over 8,500 RVA systematically retrieved from rotavirus database NCBI Virus Variation Resource. In line with previous designations, using pairwise comparison cogent nucleotide stringent bootstrap support, reference lineages defined. This study proposes provides dataset ranging 34 145 for each gene orderly global RVAs detected human animals. The identified five 31 depending gene. least number (five seven) observed genotypes A2 (NSP1), T2 (NSP3) H2 (NSP5) which are limited whereas most (31) was E2 (NSP4). Sharing same constellations backbone between recently-emerging, G8P[8] reassortants many contemporary G2P[4] provided strong support hypothesis originated primarily reassortment events recent past involving one parent ordinary 1 or animal other. selected will help researchers identify given strain belongs, trace history circulation.