Effect of radiotherapy on the expression of cardiovascular disease-related miRNA-146a, -155, -221 and -222 in blood of women with breast cancer

• DOI: 10.1371/journal.pone.0217443
• Publication Date: 2019-05-31T17:39:10Z
• Authors: Roser Esplugas; Meritxell Arenas; Noemí Serra; Montserrat Bellés; Marta Bonet; Marina Gascón; Joan-Carles Vallvé; Victoria Linares
• Keywords: Adult; Organs at Risk; Science; Breast Neoplasms; 03 medical and health sciences; 0302 clinical medicine; Humans; Radiation Injuries; Mastectomy; Aged; Aged, 80 and over; Gene Expression Profiling; Q; R; Heart; Chemoradiotherapy, Adjuvant; Middle Aged; 3. Good health; MicroRNAs; Cardiovascular Diseases; Disease Progression; Medicine; Female; Radiotherapy, Adjuvant; Dose Fractionation, Radiation; Biomarkers; Research Article

Breast cancer (BC) is one of the most important neoplasias among women. Many patients receive radiotherapy (RT), which involves radiation exposure thoracic zone, including heart and blood vessels, leading to development cardiovascular disease (CVD) as a long-term side effect. The severity CVD-related pathologies leads research on assessing novel CVD biomarkers diagnostic, prognostic or therapeutic agents. Currently, possible candidates include microRNAs (miRNAs). Previous studies have supported role for miRNA-146a, -155, -221, -222 in progression CVD. Our purpose was evaluate RT-induced modulation expression these miRNAs women with BC. Pre-RT control post-RT samples were collected, after miRNA isolation reverse transcription, levels selected measured by real-time PCR. results showed that miRNA-155 exhibited lowest expression, while miRNA-222 highest followed miRNA-221. each individual positively correlated others both pre-RT inversely age before RT. Furthermore, RT promoted overexpression miRNAs. Their also affected CVD-linked clinical parameters, treatment BC side. Modulation together other risk factors might be associated future pathologies. Further confirmatory are needed assess their potential targets patients.