MicroRNA858-mediated regulation of anthocyanin biosynthesis in kiwifruit (Actinidia arguta) based on small RNA sequencing

• DOI: 10.1371/journal.pone.0217480
• Publication Date: 2019-05-23T14:47:29Z
• Authors: Yukuo Li; Wen Cui; Ran Wang; Miaomiao Lin; Yunpeng Zhong; Leiming Sun; Xiujuan Qi; Jinbao Fang
• Keywords: 0301 basic medicine; Science; Q; Actinidia; R; High-Throughput Nucleotide Sequencing; Biosynthetic Pathways; Anthocyanins; MicroRNAs; 03 medical and health sciences; Gene Expression Regulation, Plant; RNA, Plant; Fruit; Medicine; Research Article

As important regulators, miRNAs could play pivotal roles in regulation of fruit coloring. Actinidia arguta is a newly emerged tree with extensively application prospects. However, involved A. coloring are unknown. In this study, were investigated at three developmental stages by small RNAs high-throughput sequencing. A total 482 conserved corresponding to 526 pre-miRNAs and 581 novel 619 grouped into 46 miRNA families. Target gene prediction analysis revealed that miR858, strongly candidate miRNA, was anthocyanin biosynthesis which contributes The level determined cultivars UPLC-MS/MS (ultra-performance liquid chromatography coupled tandem mass spectrometry). addition, qPCR (quantitative real-time PCR), cluster conducted as well correlation analysis. All results combined propose model describes an association arguta. data presented herein the first report on profile arguta, can provide valuable information for further research