Development of rice conidiation media for Ustilaginoidea virens

• DOI: 10.1371/journal.pone.0217667
• Publication Date: 2019-10-24T17:46:33Z
• Authors: Yufu Wang; Fei Wang; Songlin Xie; Yi Liu; Jinsong Qu; Junbin Huang; Weixiao Yin; Chaoxi Luo
• Keywords: Conidiation; Panicle; Smut

Rice false smut, caused by the ascomycete Ustilaginoidea virens, is a serious disease of rice worldwide. Conidia are very important infectious propagules U. but ability pathogenic isolates to produce conidia frequently decreases in culture, which influences pathogenicity testing. Here, we developed tissue media with leaves or panicles that stimulate conidiation virens. Among tested media, 0.10 g/ml panicle medium was most efficient for conidiation. Whereas, some leaf more effectively increased than except medium, and certain non-filtered were better their filtered counterparts. Although induced smaller, they able germinate on potato sucrose agar infect normally. The also workable inducing conidiation-defective isolates. This method provides foundation production virens will be widely applicable testing as well genetic analyses smut resistance cultivars.