Study of the potential adverse effects caused by the dermal application of Dillenia indica L. fruit extract standardized to betulinic acid in rodents

• DOI: 10.1371/journal.pone.0217718
• Publication Date: 2019-05-31T17:46:42Z
• Authors: Flávia S. Fernandes; Gustavo S. da Silva; Alexandre S. Hilel; Ana C. Carvalho; Karina V. T. Remor; Aline D. Schlindwein; Luiz A. Kanis; Daniel F. Martins; Maicon R. Kviecinski
• Keywords: Betulinic acid

This study aimed to evaluate the potential adverse effects of dermal administration Dillenia indica Linnaeus (D. indica) fruit extract in healthy rodents; was standardized betulinic acid. In initial phase, acute were evaluated on skin application site a single dose. A irritation test performed male Wistar rats (n = 8/group) receiving (50-150 mg/mL) with acid (0.5-1.5%, respectively). photosensitivity BALB/c mice 6/group) (150 mg/mL). Afterwards, other 20, male:female, 1:1) used assess systemic alterations caused by 14 daily repeated doses monitoring mortality, body morphology, behavior, nutrition status, neuromotor reactions, organ morphology and weight, blood tests. At this time, 0.5 mg/mL clobetasol as positive control. The index suggested that negligible had occurred, even when applied rat at 150 mg/mL. However, acted photosensitizer mouse skin, showing photosensitizing activity close 10 5-methoxypsoralen. Repeated no aggressiveness, piloerection, diarrhea, convulsions, or status changes. weights did not change, have their compositions. Clobetasol reduction mononuclear leukocyte numbers. general, data suggest safe rodents but indicate caution should be taken activity; addition, further explored it may useful for phototherapeutic drug development.