Whole genome characterization and evolutionary analysis of OP354-like P[8] Rotavirus A strains isolated from Ghanaian children with diarrhoea

• DOI: 10.1371/journal.pone.0218348
• Publication Date: 2019-06-14T17:39:47Z
• Authors: Susan Afua Damanka; Sabina Kwofie; Francis Ekow Dennis; Belinda Larteley ...; Chantal Ama Agbem...; Yen Hai Doan; Theophilus Korku ...; Kazuhiko Katayama; Christabel Chika ...; George Enyimah Armah
• Keywords: Lineage (genetic); Sanger sequencing

In 2010, the rare OP354-like P[8]b rotavirus subtype was detected in children less than 2 years old Ghana. this follow-up study, to provide insight into evolutionary history of genome Ghanaian strains RVA/Human-wt/GHA/GHDC949/2010/G9P[8] and RVA/Human-wt/GHA/GHM0094/2010/G9P[8] an infant a 7-month child hospitalised for acute gastroenteritis, we sequenced complete using both Sanger sequencing Illumina MiSeq technology followed by phylogenetic analysis near-full length sequences. Both possessed Wa-like/genotype 1 constellation G9P[8]b-I1-R1-C1-M1-A1-N1-T1-E1-H1. Sequence comparison inference showed that were identical at lineage level throughout 11 segments. Their VP7 sequences belonged major sub-lineage G9-lineage III whereas their VP4 cluster I. The genes study closely related Senegalese G9P[8]b strain 2009. remaining nine segments, consistently clustered together with Wa-like RVA possessing either P[8]a or mostly African origin. introduction gene stable backbone may result might propagate easily human population, potential become important public health concern, especially because it is not certain if monovalent vaccine (Rotarix) used Ghana will be efficacious against such strains. Our full genomes GHM0094 GHDC949 adds knowledge genetic make-up dynamics