The role of oxidant stress and gender in the erythrocyte arginine metabolism and ammonia management in patients with type 2 diabetes

• DOI: 10.1371/journal.pone.0219481
• Publication Date: 2019-07-17T17:30:43Z
• Authors: Martha L. Contrer...; Lourdes Sánchez-S...; Juan A. Suárez-Cu...; Marisela Olguín-M...; Martha G. Alatris...; Norberto García-G...; Lorena Orozco; Rolando Hernández...
• Keywords: Malondialdehyde

Objectives To study the differences in levels of nitrogen metabolites, such as ammonia and nitric oxide correlations existing among them both red blood cells (RBCs) serum, well possible by gender healthy subjects patients with type 2 Diabetes Mellitus (DM). Design methods This cross-sectional included 80 diagnosed DM (40 female 40 male patients) their corresponding controls paired male). We separated serum RBC determined metabolites mainly through colorimetric spectrophotometric assays. evaluated changes main catabolic by-products metabolism, (NO), malondialdehyde (MDA). Results Healthy showed a differential distribution involved NO metabolism arginine for ornithine urea formation. Patients had increased ammonia, citrulline, urea, uric acid, ornithine, RBCs, whereas level was significantly lower men DM. These findings were associated hyperglycemia, glycosylated hemoglobin (Hb A1C), RBC's MDA. Furthermore, most DM-induced alterations nitrogen-related appear to be difference capacity release these thereby causing an abrogation gender-related management subjects. Conclusions found evidence putative role extra-hepatic mechanism controlling which differs according Type promotes higher MDA levels, culminate loss seen women men.