The absence of interleukin 10 affects the morphology, differentiation, granule content and the production of cryptidin-4 in Paneth cells in mice

• DOI: 10.1371/journal.pone.0221618
• Publication Date: 2019-09-11T17:32:31Z
• Authors: Loni Berkowitz; Catalina Pardo-Roa; Gigliola Ramírez; Omar P. Vallejos; Valentina P. Seba...; Claudia A. Riedel; Manuel Álvarez-Lobos; Susan M. Bueno
• Keywords: Paneth cell; Granule (geology); Intraepithelial lymphocyte

Paneth cells (PCs) are specialized epithelial of the small bowel that contain multiple secretory granules filled with antimicrobial peptides and trophic factors, which essential for control microorganisms growth maintaining intestinal integrity. Alterations in their function associated an imbalance normal microbiota, gastrointestinal infections inflammatory processes, such as Crohn's disease (CD). One most common murine models studying CD is IL-10-/- mouse. mice when housed conventional conditions take contact commensal develop acute enterocolitis mediated by a Th1 immune response. Even though, alterations PCs related to CD, they had not been characterized yet this mouse model. Here we show specific pathogen free have aberrant large number immature at bottom crypt ileum before developing inflammation, along reduced expression Indian Hedgehog. In addition, presented cryptidin-4, heterogeneous distribution lysozyme+ granules. The maturation were modified after colonization microbiota. On other hand, depletion microbiota altered phenotype, but did normalize PCs. Our results suggest IL-10 could be necessary integrity Moreover, our help explain why response microorganisms.