Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

• DOI: 10.1371/journal.pone.0222144
• Publication Date: 2019-09-25T17:39:34Z
• Authors: Ji Hong Kim; Chae Hwan Cho; Myung Yi Ryu; Jong-Gil Kim; Sei-Jung Lee; Tae Jung Park; Jong Pil Park
• Keywords: Aptamer

Dengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed detection dengue NS1. A series amino acid-substituted synthetic rationally designed, synthesized covalently immobilized to gold sensor surface. The performance monitored via square wave voltammetry (SWV) impedance spectroscopy (EIS). Potential NS1 were chosen according dynamic current decrease in SWV experiments. Using circular dichroism, molar ellipticity (DGV BP1–BP5) determined, indicating they had mostly similar random coil structure, not totally identical. SWV, DGV BP1 selected as promising recognition peptide limit found be 1.49 μg/mL by 3-sigma rule. showed good specificity stability NS1, with low signal interference. validation detect proteins confirmed four culture broth (from serotype 4) proof-of-concept. our incorporating statistically significant difference. These results indicate strategy can potentially used antigen, fever within miniaturized portable device point-of-care testing.