A novel strategy for interpreting the T-SPOT.TB test results read by an ELISPOT plate imager

• DOI: 10.1371/journal.pone.0222920
• Publication Date: 2019-09-25T17:42:14Z
• Authors: Tae Yeul Kim; Ho Eun Chang; Seong-Wook Lee; Soo Hyun Seo; Yun Ji Hong; Jeong Su Park; Kyoung Un Park
• Keywords: ELISPOT; Analyser

The T-SPOT.TB can be read by an ELISPOT plate imager as alternative to a labor-intensive and time-consuming manual reading, but its accuracy has not been sufficiently discussed date.1,423 test results obtained from reading using microscope were compared. agreement of qualitative was assessed Cohen's kappa coefficient. relationship spot counts studied Bland-Altman analysis.The overall percent the 95.43% with coefficient 0.91. Positive maximum net count 8 borderline showed relatively high discordance. in panel A, B, nil control good, variability did increase higher counts. On basis study findings, novel strategy for interpreting proposed.To diagnostic accuracy, positive should manually confirmed. Our could practical guide laboratories build their own strategies imager.