Naïve/Effector CD4 T cell ratio as a useful predictive marker of immune reconstitution in late presenter HIV patients: A multicenter study

• DOI: 10.1371/journal.pone.0225415
• Publication Date: 2019-12-23T18:31:40Z
• Authors: Veronica Bordoni; Bruno Brando; Pierluca Piselli; Olindo Forini; Federico Enrico P...; Umberto Atripaldi; Sara Carputo; Federica Garziano; Elisabetta Trento; Giovanna D’Agosto; Alessandra Latini; Manuela Colafigli; Antonio Cristaudo; Alessandra Sacchi; Massimo Andreoni; Gabriella De Carli; Nicoletta Orchi; Sandro Grelli; Arianna Gatti; Carlotta Cerva; Antonella Minutolo; Marina Potestà; Maria Luisa Di Ma...; Francesco Ortu; Paola Selva; Laura Del Pup; Irene Guarnori; Patrizia Lorenzini; Giusy Capuano; Andrea Antinori; Chiara Agrati
• Keywords: CD4-CD8 Ratio

A significant proportion of HIV-infected patients experiencing a late diagnosis highlights the need to define immunological protocols able help clinicians in identifying at higher risk for failure. The aim study was evaluate feasibility easy cytometric tests defining effect antiretroviral treatment (cART) on homeostasis and predictive markers early immune recovery. Chronic HIV infected (n = 202) were enrolled prospective multicentric study, their profile studied before (w0) after 24 weeks (w24) using standardized flow panel. Based CD4 T cell count treatment, divided (LP: <350/mmc), intermediate (IP: 350/mmc<CD4<500/mmc) (EP: >500/mmc) presenters. In all groups, cART introduction increased CD4/CD8 ratio, naïve (CD4 CD8) CD127-expressing cells. parallel, significantly reduced effector memory cells activation (CD38+CD8 CD95+CD4 cells). Moreover, frequency Naïve Effector correlated with several parameters key associated pathogenesis HIV, thus mirroring health system. Interestingly, we identified Naïve/Effector ratio (N/EM) w0 as marker predict Specifically, LP, N/EM responder (CD4>500/mmc w24) when compared non <500/mmc w24). Finally, multivariate analysis indicates that 24w than 1.86 recovered 96 more those lower 0.46. Altogether, our data an protocol reliable useful characterization viremic identify naïve/effector new tool reconstitution potential.