Hermetia illucens in diets for zebrafish (Danio rerio): A study of bacterial diversity by using PCR-DGGE and metagenomic sequencing

• DOI: 10.1371/journal.pone.0225956
• Publication Date: 2019-12-10T18:31:31Z
• Authors: Andrea Osimani; Vesna Milanović; Andrea Roncolini; Paola Riolo; Sara Ruschioni; Nunzio Isidoro; Nino Loreto; Elena Franciosi; Kieran Tuohy; Ike Olivotto; Matteo Zarantoniello; Federica Cardinali; Cristiana Garofalo; Lucia Aquilanti; Francesca Clementi
• Keywords: Hermetia illucens

In the present research, bacterial diversity was studied during a 6-month feeding trial utilizing zebrafish (Danio rerio) fed Hermetia illucens reared on different substrates with an emphasis fish gut diversity. A polyphasic approach based viable counting, PCR-DGGE and metagenomic 16S rRNA gene amplicon target sequencing applied. Two H. groups were coffee by-products (C) or mixture of vegetables (S). Viable counts showed wide variability substrate. Illumina allowed major minor taxa to be detected. Both samples larvae their frass S substrate highest richness evenness communities, whereas (ZHC) C (ZHS) had lowest evenness. stimulating effect bioactive compounds from occurrence Lactobacillaceae Leuconostoccaceae in has been hypothesized. Zebrafish originating two trials complex microbial patterns which Actinobacteria Alteromonadales always detected, irrespective diet used. Enterobacteriaceae guts more abundant ZHS than ZHC, thus suggesting influence (chlorogenic caffeic acids) guts. ZHC higher abundance Clostridia did ZHS, likely explained by activity bacteria this class contained C. An microbiota insect-derived suggested. The presence consistently associated found diet, attesting stability core microbiota.