Flax rust infection transcriptomics reveals a transcriptional profile that may be indicative for rust Avr genes

• DOI: 10.1371/journal.pone.0226106
• Publication Date: 2019-12-12T13:39:24Z
• Authors: Wenjie Wu; Adnane Nemri; Leila M. Blackman; Ann-Maree Catanza...; Jana Sperschneider; Gregory J. Lawrence; Peter N. Dodds; David A. Jones; Adrienne R. Hardham
• Keywords: RNA-Seq; Rust (programming language)

Secreted effectors of fungal pathogens are essential elements for disease development. However, lack sequence conservation among identified has long been a problem predicting effector complements in fungi. Here we have explored the expression characteristics avirulence (Avr) genes and candidate flax rust fungus, Melampsora lini. We performed transcriptome sequencing real-time quantitative PCR (qPCR) on RNA extracted from ungerminated spores, germinated isolated haustoria seedlings inoculated with M. lini isolate CH5 during plant infection. Genes encoding two categories proteins, namely Avr proteins cell wall degrading enzymes (CWDEs), were investigated detail. Analysis profiles 623 predicted secreted shows that six known (i.e. AvrM (avrM), AvrM14, AvrL2, AvrL567, AvrP123 (AvrP) AvrP4) fall within group 64 similarly expressed induced planta show peak early infection subsequent decline towards sporulation. Other this include paralogues an AvrL567 virulence allele, number putative proteins. By contrast, CWDEs into different clusters their distribution often unrelated to catalytic activity or substrate targets. These results suggest synthesis may be regulated coordinated fashion profiling-based analysis significant predictive power identification genes.