Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes

• DOI: 10.1371/journal.pone.0226192
• Publication Date: 2019-12-11T18:40:53Z
• Authors: Larissa Martins Melo; Jaqueline Poleto ...; Gabriela Lovizutt...; Gabriela Torres R...; Sidnei Ferro Costa; Leandro Encarnaçã...; Flávia Lombardi L...; Flávia de Rezende...; Paulo Sérgio Patt...; Valéria Marçal Fe...
• Keywords: 0301 basic medicine; Science; Down-Regulation; GATA3 Transcription Factor; 03 medical and health sciences; Dogs; Th2 Cells; Leukocytes; Animals; Dog Diseases; Leishmania infantum; Immunity, Cellular; Q; R; 500; Antagomirs; Antibodies, Monoclonal; Th1 Cells; Interleukin-12; Up-Regulation; 3. Good health; MicroRNAs; Medicine; Leishmaniasis, Visceral; T-Box Domain Proteins; Spleen; Research Article

Visceral Leishmaniasis is a chronic zoonosis and, if left untreated, can be fatal. Infected dogs have decreased cellular immunity (Th1) and develop potent humoral response (Th2), which not effective for elimination of the protozoan. Immune modulated by microRNAs (miRNAs), however, characterization miRNAs their possible regulatory role in spleen infected been done. We evaluated miRNA expression splenic leukocytes (SL) from naturally with Leishmania infantum developing leishmaniasis (CanL; n = 8) compared to healthy (n 4). Microarray analysis showed increased miR 21, 148a, 7 615, downregulation 150, 125a 125b. Real-time PCR validated differential 148a 615. Further, decrease 21 SL, means transfection inhibitor, IL-12 cytokine T-bet/GATA-3 ratio, parasite load on SL CanL. Taken together, these findings suggest that L. infection alters interferes immune infantum-infected dogs, placing this as therapeutic target