A long noncoding RNA acts as a post-transcriptional regulator of heat shock protein (HSP70) synthesis in the cold hardy Diamesa tonsa under heat shock

• DOI: 10.1371/journal.pone.0227172
• Publication Date: 2020-04-02T13:38:02Z
• Authors: Paola Bernabò; Gabriella Viero; Valeria Lencioni
• Keywords: Chaperone (clinical)

Cold stenothermal insects living in glacier-fed streams are stressed by temperature variations resulting from glacial retreat during global warming. The molecular aspects of insect response to environmental stresses remain largely unexplored. aim this study was expand our knowledge how a cold organism controls gene expression at the transcriptional, translational, and protein level under warming conditions. Using chironomid Diamesa tonsa as target species combination RACE, qPCR, polysomal profiling, western blotting, bioinformatics techniques, we discovered new pathway leading previously overlooked adaptive strategies stress. We obtained characterized complete cDNA sequences three heat shock inducible 70 (hsp70) two members heat-shock cognate (hsc70). Strikingly, showed that novel pseudo-hsp70 encoding putative long noncoding RNA (lncRNA) which is transcribed thermal stress, acting ribosome sponge provide post-transcriptional control HSP70 levels. its function suggest existence unexpected mechanism cope with stress: lowering pace production save energy optimize resources for recovery.