Proteomic characterization and comparison of ram (Ovis aries) and buck (Capra hircus) spermatozoa proteome using a data independent acquisition mass spectometry (DIA-MS) approach

• DOI: 10.1371/journal.pone.0228656
• Publication Date: 2020-02-13T13:44:51Z
• Authors: Wen Zhu; Xiao Cheng; Chunhuan Ren; Jiahong Chen; Yan Zhang; Yale Chen; Xiaojiao Jia; Shijia Wang; Zhipeng Sun; Renzheng Zhang; Zijun Zhang
• Keywords: Capra hircus; Proteome; Capacitation; Ovis

Fresh semen is most commonly used in an artificial insemination of small ruminants, because low fertility rates frozen sperm. Generally, when developing and applying assisted reproductive technologies, sheep goats are classified as one species. In order to optimize sperm cryopreservation protocols goat, differences proteomes between ram buck necessary investigate, which may contribute function spermatozoa. the current work, a data-independent acquisition-mass spectrometry proteomic approach was characterize make comparison (Ovis aries) (Capra hircus) proteomes. A total 2,109 proteins were identified spermatozoa, with 238 differentially abundant proteins. Proteins spermatozoa mainly involved metabolic pathways for generation energy diminishing oxidative stress. Specifically, there greater abundance related immune protective capacity activities ram, while protein that inhibit capacitation shows buck. Our results not only provide novel insights into characteristics potential proteins, but also expand direction