Using liquid chromatography mass spectrometry (LC-MS) to assess the effect of age, high-fat diet, and rat strain on the liver metabolome

• DOI: 10.1371/journal.pone.0235338
• Publication Date: 2020-07-01T17:33:26Z
• Authors: Greg Boyce; Mohammad Shoeb; Vamsi Kodali; Terence Meighan; Jenny R. Roberts; Aaron Erdely; Michael Kashon; James M. Antonini
• Keywords: Metabolome; Strain (injury)

The goal of this study was to use liquid chromatography mass spectrometry assess metabolic changes two different diets in three distinct rat strains. Sprague-Dawley, Fischer 344, and Brown-Norway male rats were maintained on a high-fat, or regular diet for 24 weeks. Liver tissue collected at 4, 12, weeks global small molecule metabolite using high resolution accurate coupled ultra-high-performance chromatography. results the metabolomics analysis revealed significant based both age within all Principal component that influence caused greater variation significantly changing metabolites than Brown Norway Fisher 344 strains, whereas had greatest Sprague Dawley strain only 4-week time point. As expected, involved lipid metabolism changed animals fat compared diet. There also observed concentration Tri carboxylic acid cycle intermediates extracted from liver strains showed multiple inbred more metabolically sensitive outbred strain. age, as case Dawley, is an important variable consider when assessing changes.