Microbiome diversity in Diaphorina citri populations from Kenya and Tanzania shows links to China

• DOI: 10.1371/journal.pone.0235348
• Publication Date: 2020-06-26T18:11:34Z
• Authors: Inusa J. Ajene; Fathiya M. Khamis; Barbara van Asch; Gerhard Pietersen; Brenda A. Rasowo; Fidelis L. Ombura; Anne W. Wairimu; Komivi S. Akutse; Mamoudou Sétamou; Samira Mohamed; Sunday Ekesi
• Keywords: Diaphorina citri; Candidatus

The Asian citrus psyllid (Diaphorina citri) is a key pest of Citrus spp. worldwide, as it acts vector for "Candidatus Liberibacter asiaticus (Las)", the bacterial pathogen associated with destructive Huanglongbing (HLB) disease. Recent detection D. citri in Africa and reports Las-associated HLB Ethiopia suggest that industry on continent under imminent threat. Endosymbionts gut bacteria play roles biology arthropods, especially regards to vector-pathogen interactions resistance antibiotics. Thus, we aim profile genera identify antibiotic genes within microbiome different populations worldwide citri. metagenome was sequenced using Oxford Nanopore full-length 16S metagenomics protocol, "What's my pot" (WIMP) analysis pipeline. Microbial diversity between assessed, were identified WIMP-ARMA workflow. most abundant endosymbionts ("Candidatus Carsonella", Profftella", Wolbachia). Shannon index showed from Tanzania had highest (1.92), China lowest (1.34). Bray-Curtis dissimilarity Kenya represented diverged populations, while least diverged. analyses generated 48 CARD 13 species each populations. Spectinomycin frequently found, an average 65.98% all These findings add knowledge African probable introduction source these countries.