Comprehensive genome based analysis of Vibrio parahaemolyticus for identifying novel drug and vaccine molecules: Subtractive proteomics and vaccinomics approach

• DOI: 10.1371/journal.pone.0237181
• Publication Date: 2020-08-19T17:29:01Z
• Authors: Mahmudul Hasan; Kazi Faizul Azim; Md. Abdus Shukur ...; Ishtiak Malique C...; Shah Rucksana Akh...; Md. Sorwer Alam P...; Md. Bashir Uddin; Syed Sayeem Uddin...
• Keywords: Reverse vaccinology; Proteome

Multidrug-resistant Vibrio parahaemolyticus has become a significant public health concern. The development of effective drugs and vaccines against is the current research priority. Thus, we aimed to find out drug vaccine targets using comprehensive genome-based analysis. A total 4822 proteins were screened from V. proteome. Among 16 novel cytoplasmic proteins, 'VIBPA Type II secretion system protein L' Putative fimbrial Z' subjected molecular docking with 350 human metabolites, which revealed that Eliglustat, Simvastatin Hydroxocobalamin top molecules considering free binding energy. On contrary, 'Sensor histidine kinase UhpB' 'Flagellar hook-associated 25 membrane T-cell B-cell epitope prediction, antigenicity testing, transmembrane topology screening, allergenicity toxicity assessment, population coverage analysis generate most immunogenic epitopes. Three subunit constructed by combination highly antigenic epitopes along suitable adjuvant, PADRE sequence linkers. designed constructs (V1, V2, V3) analyzed their physiochemical properties MHC molecules- results suggested V1 superior. Besides, affinity TLR-1/2 heterodimer construct could be biologically in repertoire. vaccine-receptor complex exhibited deformability at minimum level also strengthened our prediction. optimized codons was cloned into pET28a(+) vector E. coli strain K12. However, predicted further studied model animals combat associated infections.