Phenotypic and genotypic characterization of Salmonella Typhimurium isolates from humans and foods in Brazil
Galleria mellonella
Salmonella enterica
Strain (injury)
DOI:
10.1371/journal.pone.0237886
Publication Date:
2020-08-18T18:03:28Z
AUTHORS (11)
ABSTRACT
Salmonella enterica subsp. serovar Typhimurium (S. Typhimurium) causes gastroenteritis in many countries. However, Brazil there are few studies that have conducted a virulence characterization of this serovar. The aim study was to evaluate the potential S. strains isolated Brazil. Forty from humans (n = 20) and food were studied regarding their invasion survival human epithelial cells (Caco-2) macrophages (U937). Their determined using Galleria mellonella larvae model combined with analysis genes by whole genome sequencing (WGS). A total 67.5% (32.5% 35% food) invaded Caco-2 at levels similar or greater than SL1344 prototype strain. In addition, 37.5% (25% 12.5% survived U937 SL1344. (40%) (25%) showed high intermediate G. after seven days exposure. Approximately, 153 chromosomal plasmidial origin detected studied. conclusion, ability invade strain dependent not related source year isolation. rates macrophages, presented higher proportion isolates virulent profile comparison suggesting difference may be frequency which contained plasmid genes, such as spvABCDR operon, pefABCD rck mig-5.
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