Temporal transcriptome analysis suggest modulation of multiple pathways and gene network involved in cell-cell interaction during early phase of high altitude exposure

• DOI: 10.1371/journal.pone.0238117
• Publication Date: 2020-09-10T17:38:04Z
• Authors: Priya Gaur; Supriya Saini; Koushik Ray; Kushubakova Nadir...; Almaz Akunov; Abdirashit Maripov; Akpay Sarybaev; Shashi Bala Singh; Bhuvnesh Kumar; Praveen Vats
• Keywords: Gene regulatory network

High altitude (HA) conditions induce several physiological and molecular changes, prevalent in individuals who are unexposed to this environment. Individuals exposed towards HA hypoxia yields orchestration maintain adequate tissue oxygen delivery supply at altitude. This study aimed understand the temporal changes of 4,111m. Physiological parameters transcriptome was conducted high day 3, 7, 14 21. We observed differentially expressed gene (DEG) time points along with altered BP, HR, SpO2, mPAP. unsupervised learning DEG's discloses 3 as distinct point. Gene enrichment analysis ontologies pathways indicate cellular dynamics immune response involvement early exposure later stable response. Major clustering genes involved deployed into broad categories: cell-cell interaction, blood signaling, coagulation system, process. Our data reveals perturbed for like vascular remodeling, homeostasis. In we found nodal point interactive network candidate controlling many VIM, CORO1A, CD37, STMN1, RHOC, PDE7B, NELL1, NRP1 TAGLN most significant among them i.e. VIM identified top hub gene. suggests a unique perturbation likely play critical role associated pathophysiological condition during compared points.