Lower DNA methylation levels in CpG island shores of CR1, CLU, and PICALM in the blood of Japanese Alzheimer’s disease patients

• DOI: 10.1371/journal.pone.0239196
• Publication Date: 2020-09-29T18:42:58Z
• Authors: Risa Mitsumori; Kazuya Sakaguchi; Daichi Shigemizu; Taiki Mori; Shintaro Akiyama; Kouichi Ozaki; Shumpei Niida; Nobuyoshi Shimoda
• Keywords: CpG site; Apolipoprotein E

The aim of the present study was to (1) investigate relationship between late-onset Alzheimer’s disease (AD) and DNA methylation levels in six top seven AD-associated genes identified through a meta-analysis recent genome wide association studies, APOE, BIN1, PICALM, CR1, CLU, ABCA7, blood, (2) examine its applicability diagnosis AD. We examined differences at CpG island shores using Sanger sequencing, one two groups 48 AD patients elderly controls used for test or replication analysis. found that three out genes, were significantly lower subjects. combination CLU APOE genotype classified with AUC = 0.84 0.80 analyses, respectively. Our implicates onset suggests their diagnostic value.