Adenomatous polyposis coli-binding protein end-binding 1 promotes hepatocellular carcinoma growth and metastasis

• DOI: 10.1371/journal.pone.0239462
• Publication Date: 2020-09-21T20:04:56Z
• Authors: Takeshi Aiyama; Tatsuya Orimo; Hideki Yokoo; Takanori Ohata; Kanako C. Hatanaka; Yutaka Hatanaka; Moto Fukai; Toshiya Kamiyama; Akinobu Taketomi
• Keywords: Adenomatous polyposis coli

This study was performed to determine the clinical significance of adenomatous polyposis coli (APC)-binding protein end-binding 1 (EB1) in hepatocellular carcinoma (HCC) and characterize its biochemical role comparison with previous reports. We immunohistochemical staining detect EB1 expression tissues from 235 patients HCC investigated correlations clinicopathological features prognosis. also roles cell proliferation, migration, tumorigenesis vitro vivo by siRNA- CRISPR/Cas9-mediated modulation human lines. The results showed that significantly correlated several important factors associated tumor malignancy, including histological differentiation, portal vein invasion status, intrahepatic metastasis. Patients high tissue had poorer overall survival higher recurrence rates than low expression. knockdown knockout cells reduced inhibited growth vivo. Further, genes encoding Dlk1, HAMP, SLCO1B3 were differentially expressed association identified using RNA microarray analysis. In conclusion, elevated promotes metastasis HCC. may serve as a new biomarker for HCC, coexpressed represent potential targets therapy.