Ultra-rapid detection of SARS-CoV-2 in public workspace environments

• DOI: 10.1371/journal.pone.0240524
• Publication Date: 2021-02-24T18:32:35Z
• Authors: Ozlem Yaren; Jacquelyn McCarter; Nikhil Phadke; Kevin M. Bradley; Benjamin Overton; Zunyi Yang; Shatakshi Ranade; Kunal Patil; Rishikesh Bangale; Steven A. Benner
• Keywords: 2019-20 coronavirus outbreak; Work space; Betacoronavirus; Sars virus

Managing the pandemic caused by SARS-CoV-2 requires new capabilities in testing, including possibility of identifying, minutes, infected individuals as they enter spaces where must congregate a functioning society, workspaces, schools, points entry, and commercial business establishments. Here, only useful tests (a) require no sample transport, (b) minimal manipulation, (c) can be performed unlicensed individuals, (d) return results on spot much less than one hour, (e) cost more few dollars. The sensitivity need not high normally required FDA for screening asymptomatic carriers (as 10 virions per sample), these viral loads are almost certainly enough an individual to present risk forward infection. This allows specifically this trade-off unneeded necessary speed, simplicity, frugality. In some studies, it was shown that load creates forward-infection may exceed 5 milliliter, easily within RNA amplification architecture, but unattainable antibody-based architectures simply target antigens. we describe such test based displaceable probe loop architecture.