Analysis of expression of vitamin E-binding proteins in H2O2 induced SK-N-SH neuronal cells supplemented with α-tocopherol and tocotrienol-rich fraction

• DOI: 10.1371/journal.pone.0241112
• Publication Date: 2020-11-24T22:48:53Z
• Authors: Aishatu Ali Chiroma; Huzwah Khaza’ai; Roslida Abd. Hamid; Sui Kiat Chang; Zainul Amiruddin ...; Zaida Zainal
• Keywords: Tocotrienol; alpha-Tocopherol

Natural α-tocopherol (α-TCP), but not tocotrienol, is preferentially retained in the human body. α-Tocopherol transfer protein (α-TTP) responsible for binding α-TCP cellular uptake and has high affinity specificity α-tocotrienol. The purpose of this study was to examine modification α-TTP together with other related vitamin E-binding genes (i.e., TTPA, SEC14L2, PI-TPNA) regulating E neuronal cells at rest under oxidative stress. Oxidative stress induced H2O2 an hour which followed by supplementation different ratios tocotrienol-rich fraction (TRF) four hours. levels were quantified determine bioavailability levels. expression PI-TPNA 0% found be positively correlated resting cells. In addition, regulation all above-mentioned affect distribution It observed that, increased secretion occur Thus, our results showed that conclusion proteins may modified absence produce tocotrienols (TCT), as a source E. current suggests transport influence concentrations