Comparative RNA-Seq analysis unfolds a complex regulatory network imparting yellow mosaic disease resistance in mungbean [Vigna radiata (L.) R. Wilczek]

• DOI: 10.1371/journal.pone.0244593
• Publication Date: 2021-01-14T17:01:44Z
• Authors: Uttarayan Dasgupta; Gyan Prakash Mishra; Harsh K. Dikshit; Dwijesh C. Mishra; Tejas Bosamia; Anirban Roy; Jyotika Bhati; Priti; Muraleedhar Aski; Ranjeet R. Kumar; Amit Kumar Singh; Atul Kumar; Subodh K. Sinha; Shiksha Chaurasia; Shelly Praveen; Ramakrishnan M. Nair
• Keywords: WRKY protein domain; Jasmonic acid; MYB

Yellow Mosaic Disease (YMD) in mungbean [ Vigna radiata (L.) R. Wilczek] is one of the most damaging diseases Asia. In northern part India, YMD caused by Mungbean India Virus (MYMIV), while southern this (MYMV). The molecular mechanism resistance remains largely unknown. study, RNA-seq analysis was conducted between a resistant (PMR-1) and susceptible (Pusa Vishal) genotype under infected control conditions to understand regulatory network operating mungbean-YMV. Overall, 76.8 million raw reads could be generated different treatment combinations, mapping rate per library reference genome varied from 86.78% 93.35%. MYMIV showed very complicated gene network, which begins with production general PAMPs (pathogen-associated patterns), then activation various signaling cascades like kinases, jasmonic acid (JA) brassinosteroid (BR), finally expression specific genes (like PR-proteins, virus R-gene proteins) leading response. function WRKY, NAC MYB transcription factors imparting against established. string also revealed role proteins involved kinase, viral movement phytoene synthase activity resistance. A set novel stress-related EST-SSRs are identified RNA-Seq data may used find linked genes/QTLs Also, 11 defence-related transcripts validated through quantitative real-time PCR analysis. networks have led an insight about defence infection will immense use manage mungbean.