Genome-wide identification and expression profiling of glutathione S-transferase family under multiple abiotic and biotic stresses in Medicago truncatula L.

• DOI: 10.1371/journal.pone.0247170
• Publication Date: 2021-02-20T02:33:57Z
• Authors: Md. Soyib Hasan; Vishal Singh; Shiful Islam; Md. Sifatul Islam; Raju Ahsan; Amita Kaundal; Tahmina Islam; Ajit Ghosh
• Keywords: Medicago truncatula; Glutathione S-transferase

Glutathione transferases (GSTs) constitute an ancient, ubiquitous, multi-functional antioxidant enzyme superfamily that has great importance on cellular detoxification against abiotic and biotic stresses as well plant development growth. The present study aimed to a comprehensive genome-wide identification functional characterization of GST family in one the economically important legume plants— Medicago truncatula . Here, we have identified total ninety-two putative MtGST genes code for 120 proteins. All these members were classified into twelve classes based their phylogenetic relationship presence structural conserved domain/motif. Among them, 7 gene pairs segmental duplication. Expression profiling transcripts revealed high level organ/tissue-specific expression most developmental stages anatomical tissues. MtGSTU 5, 8, 17, 46, 47 showed significant up-regulation response various stresses. Moreover, 14, 28, 30, 34, 46 MtGSTF 8 found be highly upregulated drought treatment 24h 48h. stress-responsive members, MtGSTU17 strong affinity towards its conventional substrates reduced glutathione (GSH) 1‐chloro‐2,4‐dinitrobenzene (CDNB) with lowest binding energy of—5.7 kcal/mol -6.5 kcal/mol, respectively. Furthermore, substrate-binding site residues conserved. These findings will facilitate further evolutionary