Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum

• DOI: 10.1371/journal.pone.0247560
• Publication Date: 2021-03-11T18:44:20Z
• Authors: Valéria da Costa ...; Artur Augusto Vel...; Luiz Claudio Ferr...; Tatiana Machado Q...; Shanna Araujo dos...; Fabiano Borges Fi...; Monique Paiva Campos; Francisco das Cha...; Raquel de Vasconc...; Elba Regina Sampa...; Tatiana Rozental; Raphael Gomes da ...; Maria Regina Reis...; Rayane Teles-de-F...; Rafaela Vieira Bruno; Fernanda Nazaré M...; Luciana de Freita...; Rodrigo Caldas Me...
• Keywords: Parasite load; Ehrlichia canis

In canine leishmaniosis caused by the protozoan Leishmania infantum, little is known about how co-infections with or co-seropositivities for other pathogens can influence aggravation of this disease. Therefore, objectives study were to evaluate frequency certain in dogs seropositive L. infantum and their relationship clinical signs, histological changes load. Sixty-six infantum-seropositive submitted examination, collection blood bone marrow, culling, necropsy. Antibodies against Anaplasma spp., Borrelia burgdorferi sensu lato, Ehrlichia spp. Toxoplasma gondii Dirofilaria immitis antigens investigated serum. Samples from different tissues histopathology immunohistochemistry detection T. gondii. Quantitative real-time PCR was used assess load spleen samples. For Coxiella burnetii, conventional nested performed using marrow All 66 tested positive qPCR and/or culture. Fifty (76%) co-seropositive at least one pathogen: (59%), (41%), (18%). Clinical signs observed 15 (94%) monoinfected 45 (90%) pathogens. The skin did not differ significantly between dogs. number inflammatory cells higher spleen, lung mammary gland mitral valve These results suggest that infected are common region studied. However, aggravate load, although they associated a more intense reaction some organs.