Prevalence of pfk13 and pfmdr1 polymorphisms in Bounkiling, Southern Senegal

• DOI: 10.1371/journal.pone.0249357
• Publication Date: 2021-03-26T17:37:13Z
• Authors: Ambroise Ahouidi; Rafael Oliveira; Lis Lobo; Cyrille Diedhiou; Souleymane Mboup; Fatima Nogueira
• Keywords: Sanger sequencing

Background Delayed Plasmodium falciparum parasite clearance has been associated with Single Nucleotide Polymorphisms (SNPs) in the kelch protein propeller domain (coded by pfk13 gene). SNPs multidrug resistance gene 1 ( pfmdr1) are multi-drug including combination artemether-lumefantrine. To our knowledge, this is first work providing information on prevalence of k13-propeller and pfmdr1 mutations from Sédhiou, a region south Senegal. Methods 147 dried blood spots filter papers were collected symptomatic patients attending hospital located Bounkiling City, Sédhiou Region, Southern All samples between 2015–2017 during malaria transmission season. Specific regions analyzed using PCR amplification Sanger sequencing. Results The majority parasites (92.9%) harboured wild type sequence 6 synonymous changes. Regarding , wild-type alleles represented except at codon 184. Overall, 86Y was 11.9%, 184F 56.3% 1246Y 1.5%. mutant allele decreased 73.7% 2015 to 40.7% 2017. haplotype NFD 71.4% 20.8% Conclusions This study provides description genes variations Bounkiling, city Region Senegal, contributing closing gap anti-malaria drug molecular markers southern