From viability to cell death: Claims with insufficient evidence in high-impact cell culture studies

• DOI: 10.1371/journal.pone.0250754
• Publication Date: 2022-02-22T18:22:57Z
• Authors: Ali Burak Özkaya; Caner Geyik
• Keywords: Harmony (Music); Viability assay

Reliability of preclinical research is critical concern. Prior studies have demonstrated the low reproducibility results and recommend implementing higher standards to improve overall quality robustness research. One understudied aspect this issue harmony between hypotheses experimental design in published work.In study we focused on highly cited cell culture investigated whether commonly asserted claims such as viability, cytotoxicity, proliferation rate, death apoptosis are backed with sufficient evidence or not. We created an open access database containing 280 by 103 different high-impact articles well study. Our findings revealed that only 64% all were sufficiently supported there concerning misinterpretations considering tetrazolium salt reduction assays indicators apoptosis.Our analysis a discordance way they presented discussed manuscripts. To pre-clinical research, require clear nomenclature which distinctively categorized; materials methods sections be written more meticulously; selected utilized carefully. In paper for methodology collecting support those claims.