In vivo study on the healing of bone defect treated with non-thermal atmospheric pressure gas discharge plasma

• DOI: 10.1371/journal.pone.0255861
• Publication Date: 2021-10-11T19:30:51Z
• Authors: Akiyoshi Shimatani; Hiromitsu Toyoda; Kumi Orita; Yoshihiro Hirakawa; Kodai Aoki; Jun-Seok Oh; Tatsuru Shirafuji; Hiroaki Nakamura
• Keywords: Microplasma

Medical treatment using non-thermal atmospheric pressure plasma (NTAPP) is rapidly gaining recognition. NTAPP thought to be a new therapeutic method because it could generate highly reactive species in an ambient atmosphere which exposed biological targets (e.g., cells and tissues). If plasma-generated stimulate bone regeneration, can provide opportunity regenerative medicine. Here, we investigated the impact of on regeneration large defect New Zealand White rabbits simple (helium microplasma jet). We observed recovery progress defects by X-ray imaging over eight weeks after surgery. The results showed clear difference occupancy among groups with different times, whereas was not substantial untreated control group. According micro-computed tomography analysis at weeks, most successful achieved time 10 min, wherein volume 1.51 times larger than that Using H&E Masson trichrome stains, nucleated were uniformly observed, no inclusion confirmed, respectively, treatment. concluded critical filled bone. Overall, these suggest promising for fracture