Intestinal organoid-based 2D monolayers mimic physiological and pathophysiological properties of the pig intestine

• DOI: 10.1371/journal.pone.0256143
• Publication Date: 2021-08-23T17:43:25Z
• Authors: Pascal Hoffmann; Nadine Schnepel; Marion Langeheine; Katrin Künnemann; Guntram A. Grassl; Ralph Brehm; Bettina Seeger; Gemma Mazzuoli-Weber; Gerhard Breves
• Keywords: Organoid; Cholera toxin; Intestinal epithelium

Gastrointestinal infectious diseases remain an important issue for human and animal health. Investigations on gastrointestinal are classically performed in laboratory animals leading to the problem that species-specific models scarcely available, especially when it comes farm animals. The 3R principles of Russel Burch were achieved using intestinal organoids porcine jejunum. These seem be a promising tool generate vitro epithelium. 3D Organoids grown extracellular matrix characterized by qPCR. also seeded permeable filter supports order 2D epithelial monolayers. organoid-based monolayers morphologically investigated regarding their potential study physiological transport properties pathophysiological processes. They showed monolayer structure containing different cell types. Moreover, functional activity was demonstrated increasing transepithelial electrical resistance over 18 days active glucose chloride secretion. Furthermore, confronted with cholera toxin derived from Vibrio cholerae as proof concept. Incubation led increase short-circuit current indicating enhanced secretion, which is typical characteristic infections. Taken this together, our model allows investigation mechanisms focusing small intestine pigs. This line principle reduction classical experiments.