Protective and proliferative effect of Aesculus indica extract on stressed human adipose stem cells via downregulation of NF-κB pathway

• DOI: 10.1371/journal.pone.0258762
• Publication Date: 2021-10-22T20:24:27Z
• Authors: Hamzah Khawaja; Numan Fazal; Faiza Yaqub; Muhammad Rauf Ahmad; Muzaffar Hanif; Muhammad Amin Yousaf; Noreen Latief
• Keywords: 0301 basic medicine; Cell biology; NFKB1; Science; Phytochemicals; Anti-Inflammatory Agents; Aesculus; Down-Regulation; Adipose tissue; FOS: Basic medicine; Plant Science; Signal transduction; Biochemistry; Gene; NF-κB; Agricultural and Biological Sciences; 03 medical and health sciences; Cell Movement; Biochemistry, Genetics and Molecular Biology; Humans; Molecular Biology; Biology; Cells, Cultured; Cell Proliferation; Inflammation; Stem cell; Biological Activities of Prenylated Flavonoids; Plant Extracts; Superoxide Dismutase; Q; R; NF-kappa B; Life Sciences; Mesenchymal Stem Cells; Downregulation and upregulation; Iodoacetic Acid; 3. Good health; Oxidative Stress; Chemistry; Adipose Tissue; Bioavailability and Health Effects of Curcumin; Medicine; Molecular Medicine; Transcription factor; Research on Citrus Flavonoids and Health Benefits; Research Article; Signal Transduction

Inflammatory microenvironment after transplantation affects the proliferation and causes senescence of adipose-derived mesenchymal stem cells (hADMSCs) thus compromising their clinical efficacy. Priming with herbal extracts is considered very promising to improve viability in inflammatory milieu. Aesculus indica (A. indica) used treat many diseases Asia for decades. Herein, we explored protective role A. extract on human Mesenchymal Stem Cells against Monosodium Iodoacetate (MIA) induced stress vitro. ameliorated injury as depicted by significantly enhanced proliferation, viability, improved cell migration superoxide dismutase activity. Furthermore, reduced lactate dehydrogenase activity, reactive oxygen species release, senescent apoptotic were detected primed hADMSCs. Downregulation NF-κB pathway associated genes, p65/RelA p50/NF-κB 1, Interleukin 6 (IL-6), 1 (IL-1β), Tumor necrosis factor alpha (TNF-α) matrix metalloproteinase 13 (MMP-13) observed hADMSCs compared stressed Complementary gene expression, priming release transcription p65, inhibitory-κB kinase (IKK) α β, IL-1β TNF-α proteins expression. Our data elucidates that preconditioning rescued oxidative therapeutic potential relieving inflammation through regulation pathway.