Biofabrication of ZnO nanoparticles using Acacia arabica leaf extract and their antibiofilm and antioxidant potential against foodborne pathogens

• DOI: 10.1371/journal.pone.0259190
• Publication Date: 2022-01-05T18:36:50Z
• Authors: Sumreen Hayat; Asma Ashraf; Muhammad Zubair; Bilal Aslam; Muhammad Hussnain...; Mohsin Khurshid; Muhammad Saqalein; Arif Muhammad Khan; Ahmad Almatroudi; Zilursh Naeem; Saima Muzammil
• Keywords: Salmonella enterica

Emergence of multidrug resistant pathogens is increasing globally at an alarming rate with a need to discover novel and effective methods cope infections due these pathogens. Green nanoparticles have gained attention be used as efficient therapeutic agents because their safety reliability. In the present study, we prepared zinc oxide (ZnO NPs) from aqueous leaf extract Acacia arabica . The produced were characterized through UV-Visible spectroscopy, scanning electron microscopy, X-ray diffraction. vitro antibacterial susceptibility testing against foodborne was done by agar well diffusion, growth kinetics broth microdilution assays. Effect ZnO NPs on biofilm formation (both qualitatively quantitatively) exopolysaccharide (EPS) production also determined. Antioxidant potential green synthesized detected DPPH radical scavenging assay. cytotoxicity studies performed HeLa cell lines. results revealed that diameter zones inhibition found 16–30 nm, whereas values MIC MBC ranged between 31.25–62.5 μg/ml. Growth bactericidal after 3 hours incubation 2 × for E coli while S aureus enterica reached MIC, 4 8 MIC. 32.5–71.0% observed formation. Almost 50.6–65.1% (wet weight) 44.6–57.8% (dry EPS decreased treatment sub-inhibitory concentrations nanoparticles. Radical increased in dose dependent manner value 19.25 73.15%. Whereas non-toxic nature tested. study suggests can substitute chemical drugs antibiotic