Genomic miscellany and allelic frequencies of Plasmodium falciparum msp-1, msp-2 and glurp in parasite isolates

• DOI: 10.1371/journal.pone.0264654
• Publication Date: 2022-03-08T18:46:23Z
• Authors: Ibrar Ullah; Asifullah Khan; Muhammad Israr; Mohibullah Shah; Sulaiman Shams; Waliullah Khan; Muzafar Shah; Muhammad Siraj; Kehkashan Akbar; Tahira Naz; Sahib Gul Afridi
• Keywords: Merozoite surface protein; Multiplicity of infection; genomic DNA

The genomic miscellany of malaria parasites can help inform the intensity transmission and identify potential deficiencies in control programs. This study was aimed at investigating miscellany, allele frequencies, MOI P. falciparum infection.A total 85 confirmed isolates out 100 were included this that collected from patients aged 4 months to 60 years nine districts Khyber Pakhtunkhwa Province. Parasite DNA extracted 200µL whole blood samples using Qiagen extraction kit following manufacturer's instructions. polymorphic regions msp-1, msp-2 glurp loci genotyped nested PCR followed by gel electrophoresis for amplified fragments identification subsequent data analysis.Out infections detected, 30 msp-1 32 alleles specific. Successful amplification occurred 88.23% (75/85) 78.9% (67/85) 70% (60/85) gene. In K1 allelic family predominantly prevalent as 66.66% (50/75), RO33 MAD20. frequency with single infection having only K1, MAD20 21.34% (16/75), 8% (6/75), 10.67% (8/75), respectively. msp-2, both FC27 3D7 families revealed almost same frequencies 70.14% (47/67) 67.16% (45/67), Nine RII region identified isolates. overall mean multiplicity msp genes 1.6 1.8 1.4 while 1.03. There no significant association between age groups (Spearman's rank coefficient = 0.050; P 0.6) parasite density correlated marker.The showed high genetic diversity multiple clones Pakhtunkhwa, Pakistan. present genotype may provide valuable information essential monitoring impact eradication efforts region.